therefore you can choose to have FlowJo transform data display to improve visualization Platform.ï¿½ Often, compensation causes data to be compressed onto the bottom or left axis in displays.ï¿½ This can be un-aesthetic. The next section relates to the Compensation this option is designed only to control the calculation of statistics for display in the workspace window. They are requested by the Table Editor or Layout Editor. The latter option will cause statistics to be computed only if the sample has already been read into memory (and still resides there).ï¿½ FlowJo will always compute the statistics once ï¿½ You can also choose to have the statistics computed only The default behavior is to compute immediately (which may cause FlowJo to need to load the sample data). This setting will permit you to postpone recalculation on some or all samples to maintain the responsiveness of the program. The popup menu for Adding Analyses to the Workspace controls how FlowJo causes recalculation of gates & statistics that are copied between subsets (or samples) to occur.ï¿½ Depending on how large your experiment is, it may becomeĬumbersome to have FlowJo recalculate all populations affected by the action of dragging a population to a group or sample. The workspaces are named with incrementing version numbers (i.e., name.jo-1, name.jo-2 etc.) when the Always use the same answer box is checked upon the first save. Save with Incrementing Version Number saves a new workspace every time FlowJo saves your work. Lets you choose to save or not to save the workspace This dialog has a checkbox that lets you specify to always use the same answer:ï¿½ if you check this box and click Yes, then FlowJo will automatically save the workspace for you

When this amount of time has elapsed, FlowJo puts up a dialog and By entering a non-zero value in the box, you instruct FlowJo to remind you to save the workspace every few minutes. The next option is to Remind to Save your work. Use As Default, FlowJo records the columns & spacing that you have set in the current workspace, and uses that for all new workspaces that you create. The final button in the section is to set the order and size of columns visible in the workspace.

There you can select multiple criteria from among the data stored in the fcs file, sorting first by $DATE then by $ETIM for example. If you want a different sort order, return to the workspace and select Sort.

Annotation such as Sample ID or Patient ID can be entered while acquiring your dataĭefine Sort Order lets you choose whether data will be sorted by sample name or acquisition date and time. For instance, displaying Sample ID or Patient ID as the file name may aid in identifying your samples. 001 suffix) rather than using the internally-defined keyword.Īn alternative file naming option is to Specify This option displays the name as it is on the system disk (i.e. To avoid this problem, choose the next option, Use Data File Name. NOTE: Options chosen when acquiring your data can cause all the samples in the workspace to be named identically (i.e., each file name is missing the suffix. When you choose to display the sample's file name from the Sample Name Keyword - FlowJo examines the FCS keyword header informationĪnd uses the sample name keyword from the data file as the file name in the workspace. Just below, you can choose how FlowJo will display the data file names in the workspace window list. When FlowJo opens, it opens a new workspace window as well. Unchecked, the number will reflect the portion of all cells in the Of Parent allows you to specify that the percentage shown in the Statistic column of the workspace refers to the portion of the immediate parent population.

Further options let you choose to have FlowJo display a light grid over the sampleĪnd group list to aid in visualization.ï¿½ The setting Gate Statistic is Freq. The time counter is shown in the tool bar of the workspace window. Time Since Last Save to show the amount of time that has past since you saved this workspace. The section marked Appearance defines preferences about the how the workspace appears.

Flowjo envoy workspace